Yes, it can. Auslogics Disk Defrag supports the following Windows versions:
Windows XP with SP2 or later (32-bit and 64-bit)
Windows 2003 (32-bit and 64-bit)
Windows Vista (32-bit and 64-bit)
Windows 2008 (32-bit and 64-bit)
Windows 7 (32-bit and 64-bit)
It does not support Windows 2000, 95, 98, 98SE or Windows ME.